what is a plasmid quizlet

An agent used to transfer DNA in genetic engineering. -for example, F plasmid has been parasitised by transposon -this transposon is capable of directing a transposition event where F-plasmid integrates into another plasmid to form a co-integrate-resolution after conjugative transfer but both plasmids now have the transposon Plasmid, in microbiology, an extrachromosomal genetic element that occurs in many bacterial strains.Plasmids are circular deoxyribonucleic acid (DNA) molecules that replicate independently of the bacterial chromosome.They are not essential for the bacterium but may confer a selective advantage. DNA is released via a T4SS encoded by a genetic islandTransposable elements are sequences of DNA that are able to transpose to new positions within the genome of a single cellTrue/False: Transposition is usually an inter-molecular eventThe pathway highlights the fact that in the process of moving, there is a replication of TE through the act of replication, both cells have a copyDefine the phase 'TE's act as 'hot-spots' for recombination events'They can transpose, form co-integrates, can cause deletions (& inversions) of DNA adjacent to the TEConduction is Tn-mediated mobilisation of a non-conjugative plasmidWhat does 'DNA deletion' and 'DNA inversion' events relate to?A single gene (int) that codes for a recombinate enzyme (integrase)What does 'gene capture and expression system' and a 'gene release system' relate to?Circular DNA encoding a single, promoterless gene and an attc sequenceWhat are the 6 features of 'pathogenicity islands'?True or false: PAI's are examples of a genomic islandWhat are the 4 features of prokaryotic plasmid vectors?Some plasmids have replicons that allow them to replicate in bacteria other than E. coliConjugation can be carried out using a plasmid known as a mobilisable plasmid vectorA plasmid that has a narrow host range replicon systemWhat are the steps in 'targeted gene disruption using an SV plasmid'1. This also ensures that the gene of interest is increased during genomic division.

Plasmids are autonomous molecules and exist in cells as extrachromosomal genomes, although some plasmids can be inserted into a bacterial chromosome, where they become a permanent Bacterial conjugation (classical Tra (transfer) systems)What are the Neisseria gonorrhoeae DNA release systems?1. a small, circular, double stranded DNA molecule, which can replicate independently from its chromosomal DNA. If plasmids are used for experiments, they are called vectors.because genes in plasmids often provide the bacteria with genetic advantages, including for example antibiotic resistance. Isolate plasmid DNA and tag with a 'signature' sequence

Start studying pGLO. Sex pilus attaches to receptors on the recipient cellWhat is the process that involves the formation of sex pili and a channel through which DNA passes from donor to recipient?Proteins locate a particular DNA sequence, form a nucleoprotein complex, cut DNA to start process of DNA transfer from donor to recipientInvolves traG protein that aids transfer of plasmid, where the end result is both donor and recipient containing the plasmidFinO codes for a repressor that inhibits traJ that is needed to switch on genesStreptococcus, Enterococcus, Staphylococci, BacillusTwo strains of bacterium, each with a plasmid and that produce pili, is it possible for the two strains to conjugate?Fertility inhibition refers to a phenomenon where when one plasmid is in a cell with another, it can block the fertility function of one of the plasmidsWhen in the same cell, R100 blocks transfer of F (reduced the fertility of F).

The ideal plasmid vectors have high copy numbers inside the cell. Start studying Plasmids.